Production of monoclonal antibody against human nestin
AJMB-Avicenna Journal of Medical Biotechnology. 2010; 2 (2): 69-77
em Inglês
| IMEMR
| ID: emr-123752
ABSTRACT
We have employed a peptid-based antibody generation protocol for producing antibody against human nestin. Using a 12-mer synthetic peptide from repetitive region of human nestin protein devoid of any N-or O-glycosylation sequences, we generated a mouse monoclonal antibody capable of recognizing human, mouse, bovine, and rat nestin. A wide variety of nestin proteins ranging from 140-250 kDa was detected by this antibody. This antibody is highly specific and functional in applications such as ELISA, flow cytometry, immunocytochemistry, and Western blot assays
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Peptídeos
/
Polietilenoglicóis
/
Ensaio de Imunoadsorção Enzimática
/
Imuno-Histoquímica
/
Western Blotting
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Citometria de Fluxo
/
Hibridomas
/
Proteínas de Filamentos Intermediários
/
Proteínas do Tecido Nervoso
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Avicenna J. Med. Biotechnol.
Ano de publicação:
2010
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