miR-155 down Regulation by LNA inhibitor can reduce cell growth and proliferation in PC12 cell line
AJMB-Avicenna Journal of Medical Biotechnology. 2011; 3 (2): 61-66
em Inglês
| IMEMR
| ID: emr-124073
ABSTRACT
MicroRNAs [miRNAs] are a class of small non coding regulatory RNAs that have key functions in multiple cell processes. Deregulation of these tiny miRNAs are involved in various human diseases. MiR-155 is one of the multifunctional miRNA that its over-expression has been found to be associated with different kinds of cancer such as leukemia, breast and colon cancers. It is thought that deregulation and over-expression of this microRNA may be associated with PC12 cell proliferation. So, the aim of this study was to investigate the role of miR-155 expression on PC12 cell growth. For this reason, PC12 cells were cultured and transfected by 3 different concentration [25, 50 and 75 nmol] of either LNA anti-miR-155 or scramble antisense in 24-well plate. Then, total RNA was extracted from transfected cells. miRNA cDNAs were synthesized from isolated total RNA. In the second step, miR-155 expression level was analyzed using the quantitative real-time polymerase chain reaction [QRT-PCR]. MTT test was performed to evaluate cell viability. In the next step, apoptosis assay was assessed to investigate anti miR-155 effect on PC12 cells death. Obtained results were analyzed with t-test. MTT test revealed that cell viability of transfected cells with 75 nM of anti-miR- 155 to be reduced by half of the control and scramble groups [0.5 vs. 0.97 and 0.94]. Our data suggest that miR-155 over-expression is associated with PC12 cell growth. So, miR-155 down regulation by anti-miR-155 could open up new ways to restrain brain tumor growth, as anti-miR-155 causes PC12 cells to repress
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Regulação para Baixo
/
Linhagem Celular
/
Células PC12
/
MicroRNAs
/
Proliferação de Células
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Avicenna J. Med. Biotechnol.
Ano de publicação:
2011
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