Cytotoxicity comparison of nanosilver coated gutta- percha with Guttaflow and normal gutta-percha on L929 fibroblast with MTT assay

ABSTRACT

Gutta-percha is shown to be the most widely used root canal filing material due to its well-known low toxicity potential. A new kind of gutta-percha with nanosilver coating has synthesized by Iranian researchers and claimed to have antibacterial an antifungal properties. The aim of this study was to compare the cyotoxicity of nanosilver coated gutta-percha, GuttaFlow [GF], with normal gutta-percha [GP] on mouse fibroblast cell line L929. In this in vitro study, cytotoxicity was evaluated by fibroblast cell culture and its direct contact with the test materials. 30 specimens inserted in test tubes and cell survival fraction was estimated by MTT test after Ihour, 24 hour, and 1 week. Two way ANOVA, one way ANOVA and Tukey HSD were used for statistical analysis. After 1 hour, cytotoxicity among three 3 test materials was significantly different. Nanosilver coated gutta-percha had the highest cytotoxicity and GuttaFlow the lowest one [P<0.01]. After 24 hour, no significant differences were observed [P=0.37]. After 1 week, GuttaFlow showed significantly higher cytotoxicity than normal gutta-percha and nanosilver gutta-percha [P<0.001]. However, this difference was not significant between nanosilver and normal gutta-percha [P>0.05]. In nanosilver gutta-percha group, the most cytotoxicity was observed after 1 hour while it was decreased with time and became equal to normal gutta-percha after 24 hours and after 1 week, it reached the lowest level among 3 test materials