Evaluation of the protective effect of isoprinosine against navelbine induced oxidative stress in mice

ABSTRACT

Many studies in vitro and in vivo have shown immunomodulating and antiviral activities of Isoprinosine [inosine pranobex]. Chemotherapy means the use of a drug which is most effective at, killing cells that are rapidly dividing. Vinorelbine [Navelbine, 5 oranhydrovinblastine] is a third-generation vinca alkaloid anti-tumor drug. It is widely used in the treatment of cancer such as advanced non-small-cell lung cancer [NSCLC] and advanced breast cancer. The aim of this study was to determine the possible protective effects of Isoprinosine in bacterially infected and non infected mice at a dose level of 8.75 mg/kg/day orally every second day for 15 days under the effect of anticancer Navelbine. Mitochondrial DNA fragmentation, Lipid pereoxidation [MDA], glutathione contents [GSH], phagocytosis test and serum level of immunoglobulin G and M antibodies were evaluated, a significant increase in liver homogenate and mitochondria protein carbonyl levels was observed in Navelbine alone treated mice when compared to control group. DNA fragmentation in homogenate and mitochondria was significantly increased in all test groups when compared to control group except Isoprinosine treated animal group which showed significant decrease in DNA fragmentation. The results of this study proved the immune protective and immunomodulatory effects of Isoprinosine against Navelbine induced toxicity in Pseudomonas aeruginosa infected and non infected mice