Effects of delta-tocotrienol and polyphenol on ageing induced oxidative stress and inflammation in humans

ABSTRACT

To evaluate the synergistic effects of delta-tocotrienol and polyphenols on ageing induced oxidative stress and inflammation on healthy elderly humans. It was a randomized double blinded control trial. Total 69 healthy volunteers aged 50-78 years consisting of 47 male and 22 female were included after informed consent. All the subjects were screened at baseline and randomly grouped in A and B. Nutritional supplement [delta-tocotrienol 200mg, Quercetin 300 mg, Resveratrol 100mg] and placebo [Sucrose] were given to groups A and B subjects respectively for 4 weeks. Then subjects of two groups were crossed over for next 4 weeks. Serum total antioxidant status [TAS] and Gamma glutamyl transferase [GGT] were analyzed by using Randox kits [UK] on automatic Chemistry analyzer Selectra E. Estimation of serum Nitrate was done on ELISA by using Kit [Cayman, UK]. Serum hS-C-recative protein [CRP] was analyzed by using chemiluminescent immunometric assay kit on Immulite 1000. There was significant reduction in pre versus post median [IQR] levels in serum GGT 26 [21-34] vs 21 [19-25] U/L after nutritional supplement [p=0<0.01]. Serum CRP and Nitrate levels were also significantly decreased from 2.1[1.1-3.1] and 8.30[3.52-16.20] to 1.4[0.75-2.3] mg/L and 7.65[3.74-11.58] micro mol/L respectively [p=0<0.01]. TAS was improved from 1.45 [1.33-1.62] to 1.61 [1.46-1.70] mmol/L with nutritional supplement [p=0<0.01]. The study has demonstrated that combination of tocotrienol and polyphenol delays the ageing process by reducing the deteriorating effects of oxidative stress and inflammation