[Separation and assay of some methyl biphenyl derivatives used as angiotensin II antagonists and antihypertensives by using high performance liquid chromatography reversed phase]

ABSTRACT

Angiotensin II antagonists are major development in hypertension management in over decade, because of their excellent lower side effects and their specific action, as well as their effectiveness. So the research purposed to develop HPLC reserved phase for separation and assay of some angiotensin II antagonists, Valsartan, Losartan and Irbesartan in, raw materials and tablets. Chromatographic separation was achieved on coloun C18 using isocratic elution [at flow rate 1.5 ml/min] by using 10 mM potassium dihydrogen, phosphate buffer [pH=3] and acetonetrile [60 40 v/v] as the mobile phase and ultraviolet detector set at 254 nm. This method had been validated and accepted, then this method had applied on human serums after using solid-liquid extraction for determination therapeutic concentrations of valsartan in group of patients treated with it