Application of chemometric methods for simultaneous determination of diosmin and hesperidin in pharmaceutical preparations

ABSTRACT

Classical least-squares [CLS], principal component regression [PCR] and partial least squares [PLS] techniques were proposed for the simultaneous analysis of tablets containing diosmin and hesperidin without chemical separation procedure. The chemometric calibrations were prepared by measuring the absorbance values at the wavelengths in the spectral region 230-400 nm and by using a training set of the mixtures of both drugs in methanol. The obtained chemometric calibrations were used for the estimation of diosmin and hesperidin in samples. The numerical calculations were performed with 'MATLAB 7.5.0' software. By applying these techniques to synthetic mixtures, the mean recoveries and the relative standard deviations were found as 100.20 and 0.34, 100.01 and 0.53%, and 100.53 and 0.56% for diosmin and 100.38 and 0.67% 99.97 and 0.77%, and 100.48 and 0.70% for hesperidin, respectively. The results were compared with those obtained by applying HPLC method as a reference method. The proposed methods were successfully applied to the simultaneous determination of diosmin and hesperidin in pharmaceutical tablet formulation