Effects of glycine on haemostatic changes in stressed immobilized rats

ABSTRACT

Administration of antioxidants aimed at reducing oxidative stress induced haemostatic disorders is recommended in chronic immobilization. This study was conducted to investigate the effects of glycine supplementation on haemostatic changes in chronic immobilization stress in rats. Rats were divided into normal control group, stressed [immobilized] rats, glycine-treated group and glycine-treated +stressed [immobilized] group, Platelets aggregation, Prothrombin Time [PT], Activated Partial Thromboplastin Time [APTT], as well as plasma levels of fibrinogen [fibrinogen], Fibrinogen Degradation Products [FDP] and malondialdehyde [MDA] were assessed. Immobilization stress is associated with significant reduction of PT and APTT with significant increase in platelets aggregation as well as plasma levels of fibrinogen, FDP and MDA. On the other hand glycine supplementation for immobilized rats resulted in significant prolongation of PT and APTT with significant decrease in platelets aggregation as well as plasma levels of fibrinogen, FDP and MDA. In addition, in normal rats glycine administration induced significant reduction in platelets aggregation with prolongation of APTT. PT and plasma levels of fibrinogen and FDP were significantly changed. Chronic immobilization stress s associated with increased platelets aggregation, hypercoagulability and increased free radical production. Glycine counteracted the oxidative insult in immobilization stress and ameliorated these disorders accompanying this condition