Sero-diagnostic efficacy of antigens prepared from B. abortus and B.melitensis used for agglutination tests and IELISA

ABSTRACT

In this study, 120 bovine and ovine serum samples were collected and tested for brucellosis with Rose Bengal test [RBT], modified Rose Bengal test [mRBT], buffered acidified plate test [BAPT] using conventional Rose Bengal antigen [RBA] and buffered acidified plate antigen [BAPA] and antigens prepared from B. melitensis [the main cause of brucellosis in Egypt] strain 16-M. Indirect ELISA was used for testing the same serum samples using 4 different coating antigens which were S-LPS and OMP antigens prepared from 16-M strain and S-LPS and OMP prepared from S19 strain. There were some differences between the results of conventional Rose Bengal and BAPA antigens prepared from B. melitensis 16-M strain. In the same time, there were no characteristic differences in results of Indirect ELISA