[Development of agglutination test for detection of isolated mannoprotein antigen from candida albicans]

ABSTRACT

Candida albicans is one of the most common causes of hospital infections. The aim of this study was to develop a rapid, easy and sensitive way to detect candida albicans. Polyclonal antibodies were prepared in rabbits. The antibodies were purified by salt concentration and Ion exchange chromatography. The purified antibody was coated onto the colloidal gold. Color change [red to blue] was observed when the purified antigens [mannoprotein of Candida albicans cell wall] were added to Polyclonal antibodies. The method was sensitive and easy. This study indicated that using colloidal gold particle agglutination method can be used for accurate and rapid candida detection Method.