Development of a high-resolution melting method for screening R188h polymorphism in XRCC2 gene
IJB-Iranian Journal of Biotechnology. 2013; 11 (2): 104-108
em Inglês
| IMEMR
| ID: emr-133107
ABSTRACT
The High Resolution Melting [HRM] method is a new scanning method for detecting unknown changes in DNA and its advantages have persuaded researchers to recruit it as a screening method. Here, we developed a HRM method to screen R188H SNP [rs3218536] of XRCC2 and compared the results with a well known PCR-RFLP technique. Genomic DNA samples from 350 healthy individuals were obtained. PCR-HRM analysis and PCR-RFLP method were performed simultaneously. Three different melting profiles corresponding to three different genotypes recognized by HRM analysis. The results of PCR-RFLP showed no discrepancy. We concluded that the HRM technique can be used as a screening method for rapid discrimination of R188H genotypes in XRCC2 gene.
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Polimorfismo Genético
/
Polimorfismo de Fragmento de Restrição
/
DNA
/
Reação em Cadeia da Polimerase
/
Técnicas de Genotipagem
/
Congelamento
Tipo de estudo:
Estudo de rastreamento
Limite:
Humanos
Idioma:
Inglês
Revista:
Iran. J. Biotechnol.
Ano de publicação:
2013
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