Genetic polymorphism at the growth hormone locus in Iranian Talli goats by polymerase chain reaction-single strand conformation polymorphism [PCR-SSCP]

ABSTRACT

The growth hormone gene could be an attractive candidate gene for milk production in goats. Single-strand conformation polymorphism was used to identify polymorphism at the goat growth hormone [gGH] gene. For this purpose, genotyping of 90 Talli goat breeds was performed. Nine conformational patterns were observed in exon 4 of the gGH gene, with frequencies of 27.7% for the homozygous pattern [AA] and 72.2% for all of other heterozygous patterns [A/B, A/C, A/B/C, A/B/D/E, A/B/C/F, A/C/F, A/B/E, A/B/F]. The results showed that exon 4 of the GH gene in Talli goats is highly polymorphic