Isolation and standardization of camelpox virus from naturally infected cases in central region of Saudi Arabia 2004 [with 8 photos and one table]

ABSTRACT

Camelpox virus in skin lesion scrapings; collected from camels severely affected with clinical signs of camelpox; suspecion was detected by Indirect Fluorescent Antibody [IFA] test using standard anti Jouf-78 strain camelpox virus rabbit antiserum. The causative agent was isolated and purified throughout five successive passages on vero cell cultures using the highest positive dilutions. The virus isolate showed identical Cytopathogenic Effects [CPE] in inoculated vero cell cultures between the 1[st] and 5[th] passages. The isolate was standardized as a virulent camelpox virus by application of IFA, virus neutralization and pathogenicity tests, and designated as Saudi Arabia, Uniza 2004 strain