Differentiation of bovine spermatogonial stem cells into osteoblasts
AJMB-Avicenna Journal of Medical Biotechnology. 2011; 3 (3): 149-153
em Inglês
| IMEMR
| ID: emr-136636
ABSTRACT
Spermatogonial Stem Cell [SSC] technologies provide multiple opportunities for research in the field of biotechnology and regenerative medicine. The therapeutic use of Embryonic Stem Cells [ESCs] is restricted due to severe ethical and immunological concerns. Therefore, we need a new pluripotent cell type. Despite well-known role of germ cells in the gametogenesis, some facts apparently show their multipotentiality. In the present study, bovine SSCs were co-cultured with Sertoli cell for 7 days. Sertoli cells and SSCs were identified by Vimentin and Oct-4 immunocytochemical staining method, respectively. In order to differentiate SSCs into osteoblasts, we used consecutive inducer media without separation of the colonies. We characterized osteoblasts using Alizarin red staining
Buscar no Google
Índice:
IMEMR (Mediterrâneo Oriental)
Idioma:
Inglês
Revista:
Avicenna J. Med. Biotechnol.
Ano de publicação:
2011
Similares
MEDLINE
...
LILACS
LIS