Improved protocol for isolation of genomic DNA from leaf tissues of phyllanthus emblica gaertn

ABSTRACT

Modified Cetyltrimethylammonium bromide [CTAB] protocol for DNA isolation was developed from leaf tissues of Phyllanthus emblica for obtaining high quality genomic DNA. Fresh leaves of three different maturity were analyzed for yield and quality of DNA. Acidity was determined in three different maturity of leaves viz. tender, intermediate and mature and their influence on DNA quality was determined. Drastic reduction of pH was the primary cause for poor quality of DNA. However, high quality DNA isolation was achieved by stabilizing the pH by addition of NaOH during different stages of DNA isolation process. The present protocol yielding high quality intact DNA for genetic fingerprinting as well as for amplification of chloroplast genes for molecular analysis: