Evaluation of extended spectrum beta lactamases [ESBLs] production among Enterobacteriaceae isolates obtained from aminu Kano teaching hospital [AKTH], kano, Nigeria

ABSTRACT

Gram negative bacterial isolates in 404 gram quantity obtained from Aminu Kano Teaching Hospital [AKTH], Kano, Nigeria, were subjected to biochemical characterization using standard procedures. All confirmed Enterobacteriaceae isolates were further subjected to screening for Extended Spectrum p-Lactamases [ESBLs] production using Double Disc Synergy Test [DDST] and National Committee for Clinical Laboratory Standards [NCCLS] test. Standard discs of Augmentin [AMC 30 ng [Oxoid, England]] and Cephotaxime [Ce 30 jig], Ceftazidime [Ca 30 ug] and Ceftazidime/Clavulanic acid [Cac 30 ng] [Hi-Media, India] were used in the screening. The results of DDST showed that 45 [11.39%] were ESBLs producers viz Escherichia coli [16], Klebsiella species [14] and Proteus species [15] while that of confirmed ESBLs producers using NCCLS test was 24[6.08%]. These include; Escherichia coli [10], Klebsiella species [9] and Proteus species [5]. There exist significant differences in ESBLs production among the isolates when the results were subjected to chi-square statistical analysis. Detection of ESBLs among the clinical isolates is an indication of possible treatment failure using p-lactam antibiotics which is threatening to the current use ofp-lactam antibiotics in treatment of microbial infections