[ effect of vitamin E on diabetes induced antioxidant capacity, lipid peroxidation and nephropathy in rats]

ABSTRACT

In present study the effect of vitamin E on diabetes induced nephropathy, plasma antioxidant capacity and lipid peroxidation was investigated. 24 male wistar rats with average body weight of 250 +/- 20 gr were chosen. 16 rats, diabetized by STZ [60 mg/kg B.W, i.p] were divided into 3 groups [n=8] of non-diabetic, diabetic non-treated and diabetic treated with Vit.E [300 mg daily]. After 8 weeks all rats were anaesthetized by hydrate chloral. After blood sampling, kidneys were removed and kept in 10% formalin buffer. Plasma and red blood cells of blood samples were separated. Plasma antioxidant capacity by FRAP method, and malondialdehyde [MDA] as lipid peroxidation indicator were measured. Also renal samples were studied for focal cell proliferation and glomerule and tubule structural changes. MDA in non-diabetic, diabetic and diabetic treated with Vit.E rats was 126.14 +/- 6.6, 245.2 +/- 17 and 170.8 +/- 9 nmol/grHb respectively. Significant attenuation of MDA in diabetic treated with Vit. E rats showed decrease of lipid peroxidation in comparison to the diabetic non treated group [P<0.01]. Antioxidant capacity in the three groups mentioned was 582.7 +/- 23.33, 586.2 +/- 23.79 and 808.7 +/- 30.82 mmol/lit respectively. Plasma antioxidant capacity in Vit.E treated rats showed significant augmentation comparing to the first two groups [P<0.05]. In the non-treated rats, glomerule diffused proliferation, cell diffused inflammation and hyaline changes were seen arteries walls also showes thickening. These changes were significantly reduction in rats treated with Vit. E. This study showed that Vit. E causes decrease the oxidative effects stress and improve diabetes induced nephropathy