[Evaluation of anti tumor activity of candida albicans cell wall fraction on human fibrosarcoma cell in vitro]

ABSTRACT

Matrix metalloproteinases [MMPs] plays a role in the several physiologic and pathologic events. There is some evidence indicating the involvement of MMPs in tumor invasion and inflammatory diseases. In this study we evaluated the effect of cell wall Candida albicans fraction on MMP production by the fibrosarcoma cell line using an in vitro cytotoxicity assay, sodium dodecyl sulfate-polyacrylamide, and gelatin zymography. It was an experimental study. Fraction of cell wall Candida albicans was extracted by lysis buffer and] 0, 10, 50, 100 [micro g/ml from fraction was added to human fibrosarcoma cell line in 96 well microplate and inhibitory effect on tumor cell invasion was investigated by MTT and zymography test. Our results showed that fraction of cell wall Candida albicans was found to exhibit a inhibitory effect on tumor cell line in MTT test because the viability of tumor cells was decreased versus the control groups. Also the production of metalloproteinases enzyme in presence of fraction was diminished significantly [P<0.05]. Since inhibition of MMP activity has been employed in modality therapy in diseases such as cancer, this fraction could be promised in the preparation of anti-MMP therapeutic derivatives