HLA class I and II genotyping by PCR-SSO in patients with type-1 diabetes mellitus

ABSTRACT

Type 1 diabetes mellitus is a chronic autoimmune disease that involves destruction of the pancreatic beta cells. It is well known that both genetic and environmental factors involved in pathogenesis of type-1diabetes mellitus. This study seeks to determine whether there is any association between human leukocyte antigen class I and II alleles and type-1diabetes mellitus. Seventy type-1 diabetes mellitus patients compared to 30 apparently healthy individual were enrolled in this study. Human leukocyte antigens genotyping were analyzed by polymerase chain reaction sequence specific oligonucleotide technique. The present study revealed significantly high frequency of DQB1*0101 and DQB1*0201 alleles among patients in comparison with healthy control, while there was significantly low frequency of each HLA-A*3301, B*0826, DRB1*0701, *1101 and HLA-DQB1*0604 alleles in patients as compared to healthy controls. These findings indicate that higher frequency of HLA-DQB1*0101 and *0201 alleles may be a risk factor for type-1diabetes mellitus, meanwhile low frequency of HLA-A*3301, B*0826, DRB1*0701, *1101 and HLA-DQB1-*0604 alleles could be a protective factor