Chromatographic methods for simultaneous determination of diiodohydroxyquinoline and Metronidazole in their binary mixture

ABSTRACT

Two chromatographic methods were developed for analysis ofdiiodohydroxyquinoline [DIHQ] and metronidazole [MTN]. In the first method, diiodohydroxyquinoline and metronidazole were separated on TLC silica gel 60F254 plate using chloroform acetone glacial acetic acid [7.5 2.5 0.1, by volume] as mobile phase. The obtained bands were then scanned at 254 nm. The second method is a RP-HPLC method in which diiodohydroxyquinoline and metronidazole were separated on a reversed-phase C18 column using water methanol [6040, V/V, PH=3.6] as mobile phase at a flow rate of 0.7 mL.min[-1] and UV detection at 220 nm. The mentioned methods were successfully used for determination of diiodohydroxyquinoline and metronidazole in pure form and in their pharmaceutical formulation