[Production of Braxy vaccine by fermenter and enriched culture media]
Journal of Veterinary Research. 2013; 68 (3): 297-304
em Persa
| IMEMR
| ID: emr-140963
ABSTRACT
Braxy vaccine, a culture of a suitable strain or strains of Clostridium septicum in a fluid medium, its filtrate or derivatives, will be inactivated so that it produces its immunogenic activity without toxicity. Production of C. septicum vaccine [Braxy vaccine] using enriched culture media in fermenter. Conventional and enriched culture media were used for growing C. septicum [CN913] vaccinal strain in both 10 liter glass bottle and fermenter, in a triplicate manner. All twelve experiments were inspected to ensure compliance with the requirements of the vaccine international standards for minimum lethal dose [MLD], sterility, freedom of abnormal toxicity, safety and potency tests. Results showed that the culture of C. septicum in fermenter using enriched culture media is the best. Based on international standards, 2.5IU/mL was determined for C. septicum alpha toxin potency test, but in this study 4IU/mL was obtained. Using enriched culture media in fermenter is suitable for braxy vaccine production
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Meios de Cultura
/
Clostridium septicum
/
Fermentação
Idioma:
Persa
Revista:
J. Vet. Res.
Ano de publicação:
2013
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