Molecular detection of human papillomavirus type-16 DNA in cervical cancer tissue biopsies
Bahrain Medical Bulletin. 2013; 35 (4): 183-185
em Inglês
| IMEMR
| ID: emr-143103
ABSTRACT
The aim of the study is to detect the frequency of human papillomavirus type-16 among patients with cervical carcinoma. Khartoum Hospital, Army Medical Hospital and Soba University Hospital, Sudan. Descriptive-cross sectional study. Fifty specimens of treated cervical biopsy sections [Paraffin embedded] were included in the study from April to October 2012. DNA was extracted followed by the detection of E6 gene of human papillomavirus type-16 using non-probed SYBER green real-time PCR. Thirty [60%] showed positive results as compared with the sigmoid curve of the positive control for HPV type-16; while 20 [40%] were negative. Most of the positive results were among the age group 31-50 years. Human papillomavirus type-16 was detected in 60% of women with cervical cancer, which seems to have a strong association with cancer development.
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Papillomaviridae
/
Biópsia
/
DNA Viral
/
Carcinoma de Células Escamosas
/
Neoplasias do Colo do Útero
/
Estudos Transversais
/
Fatores de Risco
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Papillomavirus Humano 16
Tipo de estudo:
Estudo de prevalência
Limite:
Feminino
/
Humanos
Idioma:
Inglês
Revista:
Bahrain Med. Bull.
Ano de publicação:
2013
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