Immune reactivity of Brucella melitensis-vaccinated rabbit serum with recombinant Omp31 and DnaK proteins
IJM-Iranian Journal of Microbiology. 2013; 25 (1): 19-23
em Inglês
| IMEMR
| ID: emr-143248
ABSTRACT
Brucella melitensis infection is still a major health problem for human and cattle in developing countries and the Middle East. In this study, in order to screen immunogenic candidate antigens for the development of a Brucella subunit vaccine, a cytoplasmic protein [DnaK] and an outer membrane protein [Omp31] of B. melitensis were cloned, expressed in E.coli BL21 and then purified using Ni-NTA agarose. Immunized serum was prepared from a rabbit inoculated with attenuated B. melitensis. It was proved that immunized serum contains antibodies against recombinant Omp31 [rOmp31] and DnaK [rDnaK] by Western blot and ELISA assays. The results may suggest the importance of these proteins as subunit vaccines against B. melitensis as well as targets for immunotherapy
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Coelhos
/
Proteínas da Membrana Bacteriana Externa
/
Ensaio de Imunoadsorção Enzimática
/
Western Blotting
/
Vacinação
/
Chaperonas Moleculares
Limite:
Animais
Idioma:
Inglês
Revista:
Iran. J. Microbiol.
Ano de publicação:
2013
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