Pharmacological and biological evaluation of extracts from Gratiola officinalis L. [Scrophulariaceae]
Pakistan Journal of Pharmaceutical Sciences. 2012; 25 (3): 657-663
em Inglês
| IMEMR
| ID: emr-144421
ABSTRACT
The crude extract of Gratiola officinalis and its n-hexane, chloroform, ethyl acetate, n-butanol and aqueous fractions were subjected to biological [Brine Shrimp Bioassay, Insecticidal and Phytotoxicity/Cytotoxic] and neuropharmacological [Head dip, Open field Forced swimming test, Sodium pentothal induced sleep] activities. Results obtained in this study indicated that at high concentration dose [1000microg/ml], all test samples showed 60-95% phytotoxicity. In crude extract, n-butanol and aqueous fractions produced more than 85% phytotoxicity. While low concentration [10microg/ml] dose showed 25-28% phytotoxicity in all test samples. The crude extract was devoid of any effect against the growth of Callosbruchus analis and Tribolium castaneum and caused 10 mortality of Rhyzopertha dominica. n-Hexane, chloroform, ethylacetate, n-butanol and aqueous fractions caused 50, 30, 40, 10 and 20% mortality respectively of C. analis where as chloroform, ethyl-acetate, aqueous and crude extract, n-hexane, ethyl-acetate fractions also caused low mortality [10%] of Tribolium castaneum and Rhyzopertha dominica respectively. In cytotoxic assay at 1000microg/ml concentration, n-butanol fraction produced 36.7% and the crude extract produced 13.3% mortality of brine shrimp, its aqueous fraction was inactive at all concentrations. The results of head dip, open field, mobility time and Pentothal Na induced sleep indicated that crude extract, n-butanol and ethylacetate fractions of G. officinalis had mild sedative effect. However aqueous fraction was found to produce a significant decrease in motor activities and potentiated the duration of sleep
Buscar no Google
Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Artemia
/
Ratos
/
Extratos Vegetais
/
Relação Dose-Resposta a Droga
/
Inseticidas
/
Camundongos
/
Atividade Motora
Limite:
Animais
Idioma:
Inglês
Revista:
Pak. J. Pharm. Sci.
Ano de publicação:
2012
Similares
MEDLINE
...
LILACS
LIS