Flow cytometric analysis of peripheral T lymphocyte subpopulations in psoriasis

ABSTRACT

Psoriasis is a common inflammatory skin disorder, has received attention as a target for new pathogenesis and oriented therapies. Autoimmunity and T lymphocyte subsets are suggested to be involved in the development of psoriasis. The aim of this work is to asses the role of T lymphocyte subsets in the pathogenesis of psoriasis. We investigated the peripheral blood lymphocyte subpopulations obtained from psoriatic patient before and after treatment and from healthy controls, using two colour flow cytometry. We found highly significant suppression of total CD[3+] T Cells and CD[3+] CD[56+] NKT lymphocytes in psoriatic patient as compared to control. Also, we observed significant reduction of T helper cells in patients as compared to control. The highly significant reduction of CD[3+] T cell and CD[3+] CD[56+] NKT lymphocytes proved their actively involvement in the development of psoriasis