Study the nature and potentiation of immune response of tetanus toxoid

ABSTRACT

Immunoglobulin play an essential role in the body's immune system. The present study was designed to determined the antibodies [IgM, IgG and IgG [T]] against immunomodulators tetanus toxoid vaccine and ultracorn, in the serum horse. Twelve draughting horses of both sexes with average age of 4-6 years and 250 kg weight were used throughout the experiment. Horses were divided into four groups as follows The first control group represented [horses not received any vaccine or immunomodulators]; The second horses were injected I.M with 6ml of 60 LF purified concentrated tetanus toxoid vaccine and reinjected with same dose 6 weeks later. The third were injected S.C with 6 ml ultracorn and reinjected with same dose 6 weeks later. The fourth group horses were injected TM. with 6 ml of 60 LF tetanus adsorbed toxoid vaccine simultaneously with S.C. injection of 6m1 ultracorn. The horses injected purified concentrated tetanus toxoid vaccine, showed gradual increase of the serum immunoglobulin level [IgM, lgG, IgG [T]] after 1[st],2d,3rd and 4[th] weeks reached to the maximum level at 3rd weeks and then decreased up 6th weeks. While increased again after re-injection with the same dose at 8[th], 12[th] and 16[th] weeks. Also IgM, IgG and IgG [T] concentration patterns [mg/100 ml] at different intervals before and after vaccination with tetanus adsorbed toxoid vaccine and ultracorn immunopotntiator simultaneously showed more-increase of the [IgM, IgG IgG [T] after 1[st],2[nd],3[rd] and 4[th] weeks reached to the maximum level at 3 weeks and then decreased up 6 weeks. In group of horses injected with ultracorn alone showed increased of the serum immunoglobulin level [IgM, IgG, IgG [T]] at the same time intervals but less than tetanus toxoid group or tetanus toxoid with ultracorn immunopotntiator group. In conclusions the horses injected I.M with 6ml of 60 LF tetanus adsorbed toxoid vaccine simultaneously with S.C injection of 6 ml ultracorn showed maximum serum immunoglobulin level [IgM, IgG, lgG [T]]