Protective role of green tea against doxorubicin-induced oxidative stress in rats

ABSTRACT

Mitochondria are primary target involved in doxorubicin [DOX] cardiotoxicity which mediated by the induction of reactive oxygen species [ROS]. Therefore, the objective of this study was planned to investigate the effect of DOX on cardiac mitochondrial functions. The harmful effect of DOX on lysosomal membrane was also investigated. Moreover, the protective effect of green tea plus catechin [GT] against doxorubicin-induced cardiotoxicity was considered. Thirty two male adult albino rats [180-200 g] were divided into four groups [n=8] as follows [1] control group [saline solution, 1 ml 100 g[-1] body weight, i.p., for 10 days], [2] DOX group [a single dose of 10mg kg[-1] body weight, i.p. after 10 days of treatment with saline], [3] GT group [received 100 mg kg' body weight, per.os [p.o]. of GT for 10 days] and [4] DOX/GT group [received 100 mg kg[-1] body weight, p.o. of GT for 10 days prior to DOX adminstration]. Doxorubicin-induced significantly increased serum levels of lactic dehydrogenase [LDH] and creatine kinase [CK] which were reduced by GT administration. Pretreatment with GT significantly attenuated the doxorubicin induced increases in malondialdehyde [MDA], protein, carbonyl [PC] formation and lysosomal enzymes activities [P<0.05]. Doxorubicin significantly decreased GSH level, while pretreatment with GT blunted the decrease in GSH level [P<0.01]. This study suggests that green tea plus catechin has potentially protective effect against doxorubicin-induced cardiotoxicity generation. So, it may be worthy to consider the usefulness of GT as adjuvant therapy in cancer management