Protective immunity induced by co-injection of mixture of Mannan and B-glucan immunostimulant substances with the inactivated Bivalent Al-ND vaccine in broiler chickens

ABSTRACT

In the present study, the effect of a mixture of some immunostimulant substances on the immune response of broiler chicks to bivalent Al-ND vaccine was determined. Four groups of chicks were used. Group .1 was vaccinated with one dose of Al-ND vaccine and simultaneously injected with the mixture of immunostimulant substances. Group 2 was vaccinated with one dose of the bivalent AI.-ND vaccine. Group 3 was vaccinated with 2 doses of live ND vaccines [Hitchner then Lasota drains] and group 4 was kept none vaccinated control chicb. All birds were monitored weekly for the humoral and cellular immune response then challenged with the virulent NDV at 35 days of age. HI test was used for titration of antibodies for both AIV and NDV, phagocytic activity, Nitric oxide; Lysozyme activity and total antioxidant in serum were used to determine the cellular immune response of the chicks. Protective immunity induced in the vaccinated groups varied. The injected immunostimulant mixture demonstrates its effect on the immune response to the bivalent Al-ND vaccine in group 1 with 100% protection against the challenge NDV. Whereas, 80 and 60% protection were obtained in chicks vaccinated either. with Al-ND vaccine [group 2] or live ND vaccines [group 3]; respectively. The present study reports the effect of injection of some immunostimulant substances in augmentation of the immune response to the inactivated vaccines