Recent isolation and identification of equine herpes viral abortion [EHV-1] in Egypt-2007

ABSTRACT

The present study was planned to isolate and identify the local Equine herpes viral abortion [EHV-1]. So, samples [liver, lung, spleen, placenta and serum] of 5 aborted mares and their foeti were collected from a private stud in Egypt, with a history of recurrent abortion during [2005, 2006]. Attempts for isolation of the causative agent were performed on chorioallantoic membrane of SPF enibryonated chicken eggs and tissue culture [VERO cells]. The highest titre was obtained from the liver of foeti which ranged from 8-9 log10 EID50 at 3[rd] egg passage and 7-8 logio TCID50 at 5[th] tissue culture passage. These results were confirmed by different serological tests [AGPT, VN test, ELISA and IF] by using reference rabbit anti-EHV-1 sera. In addition, antibodies against EHV-1 were detected in mares sera by AGPT and ELISA