Your browser doesn't support javascript.
loading
Evaluation of a PCR assay to detect Enterococcus faecalis in blood and determine glycopeptides resistance genes: van A and van B
IJMS-Iranian Journal of Medical Sciences. 2012; 37 (3): 194-199
em Inglês | IMEMR | ID: emr-146144
ABSTRACT
Bacteremia due to Enterococcus faecalis is usually caused by strains resistant to most antibiotics. Effective management of the disease is dependent on rapid detection and characterization of the bacteria, and determination its sensitivity pattern to antimicrobial drugs. The aim of this study was to investigate a more rapid and reliable assay for simultaneous diagnosis of enterococcal bacteremia and its sensitivity pattern to antimicrobial drugs. Several bacterial suspensions with different content of two standard strains of Enterococcus faecalis resistant to vancomycin were used for inoculation to defibrinated sheep blood samples. PCR and routine assay was performed on all blood samples with different bacterial content. Routine assay and PCR for all inoculated blood samples with >/= 5 cfu/ml was positive. Mean time for PCR and routine assays was 10 hours and 5 days, respectively. PCR is a more rapid and sensitive assay for simultaneous detection and characterization for Enterococcus faecalis, and determination of its sensitivity pattern to vancomycin
Assuntos
Buscar no Google
Índice: IMEMR (Mediterrâneo Oriental) Assunto principal: DNA Bacteriano / Vancomicina / Testes de Sensibilidade Microbiana / Reação em Cadeia da Polimerase / Sensibilidade e Especificidade / Enterococcus faecalis / Farmacorresistência Bacteriana Idioma: Inglês Revista: Iran. J. Med. Sci. Ano de publicação: 2012

Similares

MEDLINE

...
LILACS

LIS

Buscar no Google
Índice: IMEMR (Mediterrâneo Oriental) Assunto principal: DNA Bacteriano / Vancomicina / Testes de Sensibilidade Microbiana / Reação em Cadeia da Polimerase / Sensibilidade e Especificidade / Enterococcus faecalis / Farmacorresistência Bacteriana Idioma: Inglês Revista: Iran. J. Med. Sci. Ano de publicação: 2012