Change in metabolic status of glutathione by palladium nitrate in blood components

ABSTRACT

This piece of research work present the toxicological impact of varied concentrations of Palladium Nitrate [Pd [NO[3][2] by changing the chemical status of glutathione and the way how glutathione plays its role in detoxification and conjugation processes of [Pd [NO[3][2] in whole blood components [plasma and Cytosolic fraction]. The impact of different concentration of [Pd [N03]2] on reduced glutathione level in whole blood component [Plasma and Cytosolic fraction] were measured spectrophotometrically following Standard Ellman's method. Compared with control sample, significant decrease in the GSH content in whole blood components [plasma and Cytosolic fraction] was obtained with various concentrations [100microM-1000microM] of Palladium Nitrate. Depleted GSH level was more pronounced with time incubation period [0-90] minutes. These finding shows that changes in the GSH status produced by palladium nitrate could either be due to palladium nitrate and glutathione [Pd-SG] complex formation or by conversion of reduce glutathione [2GSH + Pd[+2] -> GSSG]. This change in the GSH metabolic status provides information regarding the mechanism of palladium, in blood components