Relation of oxidant-antioxidant imbalance with disease progression in patients with asthma

ABSTRACT

Asthma is a chronic airway disorder which is associated to the inflammatory cells. Inflammatory and immune cells generate more reactive oxygen species in patients suffering from asthma which leads to tissue injury. To investigate the role of oxidant-antioxidant imbalance in disease progression of asthmatic patients. In this study, 130 asthmatic patients and 70 healthy controls were documented. For this malondialdehyde level, total protein carbonyls, sulfhydryls, activity of superoxide dismutase [SOD], catalase, glutathione peroxidase [GPx], total blood glutathione, and total antioxidant capacity [FRAP] were measured. Analysis of the data was done using unpaired student t test and one-way ANOVA analysis. P < 0.05 was considered significant. The present work showed that the systemic levels of MDA [4.19 +/- 0.10 nmol/ml, P < 0.001] and protein carbonyls [1.13 +/- 0.02 nmol/mg, P < 0.001] were found to be remarkably higher in asthmatic patients while protein sulfhydryls [0.55 +/- 0.01 mmol/l, P < 0.05] decreased as compared to controls [2.84 +/- 0.12 nmol/ml, 0.79 +/- 0.02 nmol/mg and 0.60 +/- 0.02 mmol/l, respectively]. We also observed decrease in activities of SOD [2047 +/- 50.34 U/g Hb, P < 0.05], catalase [4374 +/- 67.98 U/g Hb, P < 0.01], and GPx [40.97 +/- 1.05 U/g Hb, P < 0.01] in erythrocytes compared to control [2217 +/- 60.11 U/g Hb, 4746 +/- 89.94 U/g Hb, and 48.37 +/- 2.47 U/g Hb, respectively]. FRAP level [750.90 +/- 21.22 micromol/l, P < 0.05] in plasma was decreased, whereas total blood glutathione increased [0.94 +/- 0.02 micromol/l, P < 0.05] as seen in control [840.40 +/- 28.39 mol/l and 0.84 +/- 0.04 mmol/l]. This work supports and describes the hypothesis that an imbalance between oxidant-antioxidant is associated to the oxidative stress which plays a significant role in severity of the disease