Cytotoxicity evaluation of vancomycin and its complex with beta-cyclodextrin on human glial cell line

ABSTRACT

The possible cytotoxic effects of vancomycin and its complex with beta-cyclodextrin [beta-CD] on human glial cell line [CRL 8621] were studied accordingly by means of MTS assay. The cultured cells were incubated with various concentrations of vancomycin, beta-CD as well as beta-CD/vancomycin complex ranging from 4.69 to 300 ug/ml. A linear dose-dependency cytotoxicity followed by hermetic-like biphasic dose-dependence was observed after incubation period of 72 hours. In general, significant increase [p<0.001] of cell proliferation was observed at lower concentrations cells treated with beta-CD and their complex while cells treated with vancomycin. In contrary, regardless of the treatments given, significant [p<0.001] reduce in cell survival was found at higher concentrations >/= 150 micro g/ml. In particular, 50% inhibitory in vitro was achieved at the concentrations of 115.95 microg/ml [for beta-CD], 116.48 micro g/ml [for vancomycin] and 115.44 microg/ml [for beta-CD/vancomycin complex]