Over expression of a synthetic gene encoding interferon lambda using relative synonymous codon usage bias in Escherichia coli
Pakistan Journal of Pharmaceutical Sciences. 2013; 26 (6): 1181-1188
em Inglês
| IMEMR
| ID: emr-148549
ABSTRACT
Interferon Lambda [IFN- Lamda] is a type III interferon which belongs to a novel family of cytokines and possesses antiviral and antitumor properties. It is unique in its own class of cytokines; because of the specificity towards its heterodimer receptors and its structural similarities with cytokines of other classes. This renders IFN- Lamda a better choice for the treatment against many diseases including viral hepatitis and human coronavirus [HCoV-EMC]. The present study describes a computational approach known as relative synonymous codon usage [RSCU]; used to enhance the expression of IFN- Lamda protein in a eukaryotic expression system. Manually designed and commercially synthesized IFN- Lamda gene was cloned into pET-22b expression plasmid under the control of inducible T7-lac promoter. Maximum levels of IFN- Lamda expression was observed with 0.4 mM IPTG in transformed E. coli incubated for 4 hours in LB medium. Higher concentrations of IPTG had no or negative effect on the expression of IFN- Lamda. This synthetically over expressed IFN- Lamda can be tested as a targeted treatment option for viral hepatitis after purification
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Códon
/
Expressão Gênica
/
Escherichia coli
Idioma:
Inglês
Revista:
Pak. J. Pharm. Sci.
Ano de publicação:
2013
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