Frequency of enteric gram negative rods isolated from various clinical samples

ABSTRACT

To determine the frequency of gram negative rods in both nosocomial and community acquired infections in our setting. This descriptive study was conducted at the Microbiology Department of Fauji Foundation Hospital, Rawalpindi over a period of two years [April 2004 to March 2006]. All pus, urine, blood and HVS received during the study period were included. Samples without properly filled forms, dried swabs and improperly transported samples were excluded. Two thousand and ninety one Enteric Gram Negative Rods [EGNR] were isolated over a period of two years, They were most commonly found in urine samples [40.7%], followed by pus [37.1%], HVS [12.0%], sputum [6.12%] and blood [3.92%] samples. The most common EGNR isolated was Escherichia coli [49.7%] followed by Klebsiella pneumoniae [23.4%], Pseudomonas aeruginosa [19.5%] and Proteus species [1.81%]. The most commonly isolated Enteric Gram negative rod from urine samples [n 852] was E. coli [622] followed by Klebsiella pneumonia [189], Providentia [18] and Enterobacter species [13]. The most commonly isolated Gram negative rod from pus samples [n 852] was Pseudomonas aeruginosa [290] followed by E. coli [227], Klebsiella pneumoniae [18] and Proteus species. The most commonly isolated EGNR from HVS samples was E. coli [130] followed by Klebsiella pneumoniae [68] and Pseudomonas aeruginosa [51]. The most common EGNR found in sputum [n 128] was Klebsiella pneumoniae [56] followed by Pseudomonas aeruginosa [40] and E. coli [30]. In blood samples the most common organism isolated was E. coli, followed by Pseudomonas and Klebsiella.