Biotypmg of Pasieurella multocida isolates isolated from chickens in Egypt by RAPD PCR

ABSTRACT

The incidence of P. multocida isolates isolated from 50 random samples collected from chicken suffering from respiratory manifestation was 48%, the random serological classification showed 4 isolates [A5, A8, A9and D2]. RAPD-PCR using 4 random primers separately was demonstrated to characterize, identify and determine the differences in the DNA sequence among the isolates. Pasteurella multocida is one of the most important Gram-negative bacteria. It is upper respiratory tract [or pharyngeal region] commensal of many animal species. However, under predisposing factors the organism becomes the etiological agent of a wide range of economically important diseases, such as fowl cholera in poultry, hemorrhagic septicemia in cattle and buffalo, atrophic rhinitis in swine and snuffles in rabbits