Persistent oxidative stress in patients with chronic active hepatitis-C infection after antiviral therapy Failure

ABSTRACT

Oxidative stress and hepatocellular pathological changes are common associations with chronic hepatitis C virus [CHC] disease. The aim of this study was to assess serum antioxidant-oxidant [Redox] balance in patients with CHC infection before and after intake of the traditional antiviral therapy [pegylated interferon alpha-2b and oral ribavirin]. Blood samples from 50 biopsy-proven CHC patients, with no prior anti-viral treatment and persistently elevated serum transaminase levels for 6 months, as well as 15 age- and sex-matched healthy subjects were used for determination of the antioxidants reduced glutathione [GSH], superoxide dismutase [SOD], alpha tocopherol and ascorbic acid as well as lipid peroxidation [LPO] index [malondialdehyde [MDA]]. The measurements were repeated in the diseased group 25 weeks after pegylated interferon alpha-2b and ribavirin combination therapy. Serum levels of bilirubin, aspartate aminotransferase [AST], and alanine aminotransferase [ALT] were significantly higher in CHC patients than in the control group [P < 0.05]. Pretreatment serum MDA values were significantly higher in patients with CHC infection than the control group [P < 0.001], while serum antioxidant levels were significantly lower [P < 0.001]. Responders [10 patients] had lower pretreatment serum levels of MDA than non-responders [35 patients] [P < 0.001]. Both groups were comparable for the antioxidant serum levels. There was significant negative correlation between serum MDA and serum SOD, GSH, alpha tocopherol, and ascorbic acid concentrations in CHC patients. On the other hand, there was no correlation between the studied parameters and serum bilirubin, albumin, ALT, and AST. Redox imbalance was detected in patients with CHC. Responders had significantly lower levels of MDA than non-responders. Serum MDA may be used as a pretreatment predictor of response to antiviral treatment in patients with CHC