Structural characterisation of a polysaccharide from radix ranunculus ternati

ABSTRACT

A water soluble polysaccharide, HB-1, with a molecular weight of 23, 930, was isolated from radix Ranunculi ternati. by hot water extraction, ethanol precipitation, deproteination, ultrafiltration and gel-filtration column chromatography. Its sugar composition was determined by GLC as Glc, Ara, and Gal in a molar ration of 16.071 2.722 1. And the absolute configuration of Glc was identified as D. Smith degradation and methylation reaction showed the proportion of [-1]Glc [A] was about 16%, [-1]Glc[4-] [B] about 62% [1]Ara[4][2] [C] about 14%, and -[1]Gal[6]- [D] about 8%. The repetitive unit was likely composed of 3 As, 3 Cs, 13 Bs and 1 D. Together with the average molecular weight, it was predictable that HB-1 consisted of about seven of the repetitive unit. The inhibition activity of HB-1 on human glioma cell line SF188 was also measured, only to find it inactive