In-vitro callus induction and rosmarinic acid quantification in callus culture of satureja khuzistanica jamzad [lamiaceae]

In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In-vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA [0.1, 1.0, 2.0 and 5.0 mgL[-1]] solely or in combination with cytokinins BAP and KIN [1.0, 2.0 and 5.0 mgL[-1]]. B5 medium supplemented with 1.0 mgL[-1] IBA plus 5.0 mgL[-1] BAP and MS medium fortified with 2.0 mgL[-1] IBA and 2.0 mgL[-1] BAP were the most favorable media for callus formation with the highest induction rate [96%]. Maximum growth index [2.89 and 2.63] and maximum callus biomass [2.34 and 2.33 g fresh weight] were obtained from the callus cultured on B5 medium supplemented with 1.0 mgL[-1] IBA plus 5.0 mgL[-1] BAP and MS medium fortified with 1.0 mgL[-1] IBA plus 1.0 mgL[-1] KIN, respectively. Determination and quantification of RA in cultured calli were performed by HPLC UV/MS analysis. Calli induced from the plant and maintained on supplements of IBA and BAP in the absence of light produced RA 7.5% based on dry weight [DW]. No differentiation was observed in any callus during the course of this study