[Frequency of sialic acid binding adhesin gene in Helicobacter pylori isolated from patient with gastroduodenal diseases]

ABSTRACT

Sialic acid binding adhesin gene is one of the most important factors contributing to adhesin of Helicobacter pylori [H. pylori] to epithelial cell layer of stomach. The prevalence rates of sialic acid binding adhesin gene vary in different geographic areas. The aim of this study was to determine the frequency of sialic acid binding adhesin coding gene in the patients with different gastroduodenal diseases. This is a cross-sectional study. One hundred twenty patients with GI symptoms were enrolled in this study. Two gastric biopsy specimens were taken from each of the patients for rapid urease test [RUT] and DNA extraction. Presence of H. pylori was investigated by RUT and urease A gene [ureA] PCR. sialic acid binding adhesin gene was detected by using gene specific primers. Among 120 samples, presence of H. pylori was confirmed in 82 cases, of which 64 strains [78%] were positive for sialic acid binding adhesin gene. The frequency of this gene was 84.6%, 86.7%, 77.8% and 72.2% for gartric cancer, gastric ulcer, duodenal ulcer and gastritis [%66.7] respectively. The frequency of sialic acid binding adhesin gene in different samples was almost the same. Discrepancies in the frequency of this gene in different studies may be related to geographical diversity or use of different primers for detection of this gene