Identification of P fimbriae virulence factor in uropathogenic Escherichia coli by PCR in Shaherkord Hospitals

ABSTRACT

Urinary tract infection is one of the most common bacterial diseases in human being.The most important etiologic agent is Eschrichia coli and the most important virulence factor in uropathogenic E.coli is P fimberiae. Construction of P fimbriae is coded by pap operon. The importance of P fimbriae is related to the incidence of urinary tract infection, especially infection of the upper tracts. The aim of this study was to identify and determine P fimbriae prevalence in isolated uroparhogenic E.coli. A total of 182 isolates of E.coli were evaluated .Bacterial DNA was extracted by boiling method and subsequently DNA samples were examined for pap operon [pap C gene] by use of PCR method. Then PCR products were stained and identified by polyacrylamid gel electrophoresis. Based on the results of PCR, among 182 isolated strains, 66 samples [36.3%] had pap operon[pap C gene]. The results indicated that prevalence of pap operon in isolated strains was within the global range. Pyelonephritis was more common than cystitis and asympthomatic bacteriuria