Comparison of microscopical examination and semi-nested multiplex polymerase chain reaction in diagnosis of Plasmodium Falciparm and P. vivax
EMHJ-Eastern Mediterranean Health Journal. 2011; 17 (1): 51-55
em Inglês
| IMEMR
| ID: emr-158609
ABSTRACT
We compared light microscopy examination and a semi-nested multiplex PCR [SnM-PCR] assay in endemic areas of the Islamic Republic of Iran. A total of 68 individuals with malaria-positive and suspected malaria symptoms were included in the study. Giemsa-stained thick blood films were examined under a light microscope for malaria parasites in 100 and 200 fields. DNA was extracted from blood samples and SnM-PCR based on the amplification of the small subunit ribosomal RNA [ssrRNA] gene sequences was applied. Microscopical examination showed that 48.5% [33.8% P. vivax and 14.7% P.falciparum] and 50% [35.3% P. vivax and 14.7% P.falciparum] of the samples were positive in 100 and 200 fields respectively. SnM-PCR showed the same results as the 200 field microscopy
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Reação em Cadeia da Polimerase
/
Malária Vivax
/
Microscopia
Limite:
Adolescente
/
Adulto
/
Criança
/
Criança, pré-escolar
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
East Mediterr Health J.
Ano de publicação:
2011
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