L-serine dehydratase in some filamentous fungi I Studies on the formation of L-serine dehydratase by cunninghamella elegans and fusarium oxysporum

ABSTRACT

Cunninghamella elegans and Fusarium oxysporum were selected as the most potent producers of L-serine dehydratase among 16 different fungi. Of the tested metal salts, FeSO4 was the best inducer for L- serine dehydratase synthesis by C. Elegans and F. oxysporum. The enzyme was produced during the logarithmic phase of growth of the two organisms and maximum production was obtained after 3 days incubation. The optimal pH range for L-serine dehydratase formation in F. oxysporum was 4-5, whereas for C. Elegans enzyme pH 5.0 was the optimal. L-serine dehydratase of both organisms was induced with L- serine, ammonium carbonate, some amino acids and amides, but L-serine was the best induced. L-serine concentration of 2.4 gm/I was the optimal for L-serine dehydratase synthesis by both cultures. The effect of different carbon sources on enzyme formation and growth of the two organisms was investigated