[Synthesis of gold nanoparticles and study of their antimicrobial effects study on Helicobacter pylori]

ABSTRACT

Helicobacter pylori is a helix-shaped, gram-negative and microaerophilic bacterium. This bacterium is one of the main causes of peptic ulcer disease. Nowadays, multidrug therapy is used to eliminate the bacterium. Unfortunately, drug resistance to these drugs has difficulty in the treatment. The use of nanotechnology in medicine can be the solution of this problem in the future. Among nanometals, gold nanoparticles have exclusive features, which can be used in medical applications. Therefore, this study aimed to investigate the effect of gold nanoparticles on the Helicobacter pylori isolates, which are sensitive or resistant to conventional drugs. Firstly, the gold nanoparticles were synthesized by Turkevich method, and then, spectroscopic and electron microscopic characteristics were analyzed. Anti H. pylori activity of pure gold nanoparticles was determined using disk diffusion method according to CLSI standards. The size of gold nanoparticles was 10-12 nm and the maximum wavelength of the nanoparticles was obtained to be 522 nm. In the disk diffusion method, no inhibition zone was observed around gold nanoparticles discs for susceptible and resistant isolates. Findings of this study showed that low concentrations of gold nanoparticles has no effect on Helicobacter pylori isolates