[Salmonella enterica serovar Enteritidis live vaccine strain in the reproductive organs of laying goose after subcutaneous vaccination]

ABSTRACT

Serovar-speciflc real-time PCR for Salmonella enterica serovar Enteritidis [S. Enteritidis] was conducted to detect the genomic DNA of S. Enteritidis from laying goose after subcutaneous vaccination at different time points. Indirect fluorescent antibody [IFA] technique and immunohistochemical localization were employed to validate the results. The results showed that 5. Enteritidis was consistently detected in all the samples. Vagina and uterus were positive at 20 h PI, and the last organ to show a positive result was the largest and third largest preovulatory follicle, at 32 h PL The copy numbers of S. Enteritidis DNA in each tissue reached a peak at 36-60 h PI, with the vagina and uterus containing higher concentrations than other tissues. However, the number of bacteria started decreasing by 3-4 d, and by 6 d, the concentration of S. Enteritidis DNA was below the detection limits of the PCR assay, except the vagina. The real-time PCR analysis of a variety of tissues is significant for further investigation of the mechanism of vaccine protection and the optimization of vaccination regimes