Histochemical studies on the role of pantothenic acid on radiation damage of the kidney

ABSTRACT

The present study was designed to evaluate effect of pantothenic acid to gamma irradiated rats. Female albino rats [120-150gm] were subjected to [5 Gy] whole body [137]Cs gamma irradiation. Fresh kidney specimens were obtained after 1 and 4 weeks of treatment. Frozen sections were prepared for histochemical study for localization of both acid, alkaline phosphatases and succinic dehydrogenase enzymes. The enzymatic activity was quantitatively evaluated and statistically analyzed. The obtained results showed significant decrease in both alkaline phosphatase and succinic dehydrogenase enzymes activity while the acid phosphatase enzyme activity was significantly increased in comparison to the control group after one week, while after four weeks the activity of both acid and alkaline phosphatase were significantly increased, while the enzymatic activity of succinic dehydrogenase decrease. Pantothenic acid treatment [22 mg/kg body weight / day for 6 days] after half hour of radiation showed marked improvement of the radiation induced changes in the activity of measured enzymes. Finally it could be concluded that pantothenic acid could be of value in improving the radiation injury on the kidney