Determination of 18 beta-glycyrrhetinic acid in glycyrrhiza glabra l. extract by HPLC

ABSTRACT

A high performance liquid chromatography method was studied for determination of 18 p-glycyrrhetinic acid in Glycyrrhiza glabra L. [licorice] extract. The operating condition were C-18 reversed phase column [VP-ODS, [250x4.6 mm, 5 mm]] at room temperature, acetonitril/phosphoric acid [3/1] as mobile phase, at flow rate of 0.6ml/min [0-8min], 0.4ml/ min [8-20min] and UV detection at 230 nm. The recoveries were%99.60-%102.81 with relative standard deviation between%0.01-% 1.58. The relative standard deviation of the repeatability test was%2.96. The method is simple, rapid, safe, accurate, economical and useful for standardization of the licorice products