in vitro effects of graded concentrations of methanol extract of randia nilotica plant on the utilization of exogenous glucose by schistosoma mansoni worms

ABSTRACT

The efficacy of Methanol extract of R. nilotica plant in treatment of mansonial schistosomiasis in comparison with praziquantel drug in mice has been evaluated. To investigate how Randia nilotica eradicates schistosoma mansoni. The extract of Randia nilotica was obtained by a routine measurement. Cercariae of S. mansoni were obtained by infecting Biomphalaria pefeffri snails with miracidia. Fecal eggs were detected by the standard methods Mice were infected by 150 cercariae for each mouse using paddling method. The worms were recovered from those infected mice from the mesenteric and portal veins by the perfusion techniques and were incubated in Bueding medium at 37 Co. The same experiment was repeated after adding graded concentrations of the methanol extract to the medium. Quantitative analysis of glucose in the media was carried out. The mean percentage of glucose uptake by the normal worms from the medium after four hrs of incubation was 36% where as that of worms exposed to different concentrations [50 ppm, 500 ppm, 2000 ppm and 5000 ppm] of methanol extract of R. nilotica were 26%, 8%,6%,5% respectively. The inhibition of glucose uptake by this extract may be a major factor in the eradication of the worms. However, reaching that conclusion mandates prior knowledge of the effects of the R. nilotica on the enzymes and substrates of the glycolitic process of S. mansoni. The possibility of purifying an extract as a new drug against S. mansoni from this plant has to be considered