Assessment of t-cell immunity in women suffering from chronic toxoplasmosis

ABSTRACT

This study was performed to evaluate the effect of Toxoplasma gondii [T. gondii]on chosen indices of the immune response. This study involved 50 women infected with T. gondii[aged 20-45 years] proven have a chronic Toxoplasmosis [IgG positive and IgM negative]. The control group composed of 25 healthy women [aged 19 - 41 years][IgG and IgM negative] . All cases were subjected to the followingFull history taking, general and local Obstetrical and Gynecological examination, Complete blood picture, Erythrocyte Sedimentation rate [E.S.R],Renal function tests including blood urea and serum creatinine, Fasting and postprandial blood sugar, complete Liver function tests, abdominal ultrasonography and assess the rhesus factor[Rh] by agglutination test,Treponema palladium [Syphilis] by VDRLand by IHAT and agglutination test for Brucella abortus. Detection of specific anti-toxoplasma antibodies were assessed by IgG and IgM . Serum cytokines were quantitated using Sunrise ELISA reader M. code by commercially available ELISA kits for IFN y and for TNF-alpha, IL-4 and IL10 . The results of this study showed that patients infected with T. gondii had increased production of the Th-1 cytokines involved IFN y and TNF-alpha ,The mean level +/-SD of IFN y in T. gondii infection was 134.2 +/- 6.98 pg/ml.,on the other hand the mean level in healthy subjects was 24 +/- 2.99 pg/ml. [P < 0.0001] The mean level +/- SD of TNF-a in T .gondii infection was 49.1 +/- 4.1 pg/ml and in healthy subjects was 13.2 +/- 2.00 pg/ml.[P < 0 .001]. Also there is increased production of Th2 cytokines involved IL-4 and IL-10 that responsible for humoral response as compared to controls. The mean level +/- SD of IL-4 in T. gondii infection was 28.6 +/- 3.82 pg/ml and the mean level in healthy subjects was 16.1 +/- 2.4.5pg/ml. [P < 0.01]. The mean level +/- SD of IL-10 in T. gondii infection was 23.2 +/- 3.38 pg/ml .,on the other the mean level of IL-10 in healthy subjects was 11.8 +/- 2.1 pg/ml [P < 0.01] .,that responsible for the cellular response'as compared to controls group. All these subjects were living in the same geographical area with more or less similar social and economical standard