Effect of atorvastatin [ATOR] on the cardiac muscle fibres in hyperlipidemic adult male albino rats [structural and biochemical study]

ABSTRACT

Atorvastatin [ATOR] used as a cholesterol-lowering drug. Ator is one of statins widely used, commercially available and increasingly used day by day. Few studies and limited data were existed on atorvastatin induced histological and ultrastructural changes in the cardiac muscle fibres. The present study was conducted to evaluate the potential toxicity of the human equivalent therapeutic doses of Atorvastatin on cardiac muscle fibres in induced hyperlipidemic adult male albino rats. Thirty adult male albino rats were used in this study, the rats used weighing [150 +/- 10] gm. Rats were divided into groups, Group one [5 rats], served as the control group [C] .Other twenty five rats were subjected to high fat diet [25% fat and 2% cholesterol] for 3 weeks and then they subdivided into three groups. Group two [five rats] was considered as a hyperlipidemic group [H].Group three, treated group one [T1] [ten rats] received atorvastatin[Ator 20mg] [1.5 mg / day / rat]for three weeks, by the end the third week five rats were sacrificed and other five rats were considered as the recovery group one [R1], they received normal diet only for another three weeks. Group four, treated group two [T2] [ten rats] received Atorvastatin [Ator 20mg] [1.5 mg / day / rat]for other six weeks, by the end of the 6[th] week five rats were sacrificed and other five rats were considered as the recovery group two [R2], they received only normal diet for another six weeks. At the end of each step of the experiment, rats were sacrificed and specimens of cardiac muscle fibres prepared by different methods for light and electron microscopic studies. Also blood sampling was obtained forthebiochemical study. The histological data obtained from the different groups showed many changes, in hyperlipidemic group [H] indistinct and distorted